Mouse Metabolism (Core B)

Human Metabolism I  Mouse Metabolism I Genetics and Genomics

Allison Xu, PhD | Core Director

NORC members wishing to benefit from NORC support accessing these services please contact this Allison Xu: [email protected]

UCSF NORC Biomedical Mouse Metabolism Core  is specifically geared to measure a wide variety of metabolic parameters in mice, human tissues, and cultured cells. The information gained by studying mice, which are often genetically modified through use of Core C (Genetics and Genomics) and examined in conjunction with epidemiological and behavioral studies done in humans (Core A), is essential to understand mechanisms underlying metabolic disease risk, and the potential effectiveness of nutritional and pharmacologic approaches to ameliorate obesity and its consequences

Services Supported by the NORC Mouse Metabolism Core

Service Area 1: Mouse Body Composition, Food Intake, and Metabolism Facility

Provide technical support for UCSF investigators to conduct metabolic studies using a 16-chambered Comprehensive Lab Animal Monitoring System (CLAMS), a Rodent Incubator, an EchoMRI, and Dual energy X-ray absorptiometry, which together allow measurement of food intake, water intake, motor activities, core temperature, and body composition in live mice. The use of these instruments allows longitudinal measurements of key metabolic parameters in the same cohort of animals at different ages and under different feeding conditions, and will enable the accumulation of data over several months of time. We are very excited to offer access to a last generation Seahorse Bioscience XFe24 Extracellular Flux Analyzer, a platform for metabolic assays, simultaneously measuring the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a 24-well microplate, in real-time.

Service Area 2: Gnotobiotic Core Facility 

Numerous studies have shown that the microbiome has a profound effect on metabolism. The Gnotobiotics Core provides germ-free mice, fully supported gnotobiotic experiments, and germ-free re-derivation of transgenic mouse strains.