The UCSF NORC Biomedical Mouse Genetics, Metabolism & Microbiome core supports a suite of services facilitating studies in the mouse, an essential model organism in nutrition, metabolism and obesity research.
NORC members wishing to benefit from NORC support accessing these services please contact Myra Gloria (Core Administrator) and Allison Xu (Core Director).
Allison Xu, PhD | Core Director
Services Supported
Mouse body composition, food intake, and metabolism measurements
Gnotobiotic core facility
The Gnotobiotics Core provides germ-free mice, fully supported gnotobiotic experiments, and germ-free re-derivation of transgenic mouse strains to facilitate microbiome studies including studies on human transplanted microbiome.
Mouse genetic services:
Genome Engineering Core
The Facility provides ES cell targeting, genomic DNA extraction from 96-well plates, expansion of targeted ES cells, mycoplasma tests, chromosome counts, preparation of ES cells for microinjection, and CRISPR/Cas9 technologies.
Transgenenis and Blastocyte Injection Services
Provides services including microinjection of DNA for producing transgenic mice, microinjection of ES cells for producing gene knock-out mice, and 8 cell microinjection for producing 100% ES cell derived mouse embryos or mice. Transgenic Core work with UCSF ES Cell Targeting Core to provide a full-range of gene knock-out and knock-in services. Please contact the Genome Engineering Core for custom TALEN/CRISPR and ES cell targeting services.
Cryopreservation and Rederivation Services
The cryopreservation and resuscitation services allow researchers to freeze and store embryos or sperm from research lines that they no longer wish to carry but may need to resurrect for future studies.