Mouse Metabolism

UCSF NORC Biomedical Mouse Metabolism Core  is specifically geared to measure a wide variety of metabolic parameters in mice, human tissues, and cultured cells. The information gained by studying mice, which are often genetically modified through use of Core C (Genetics and Genomics) and examined in conjunction with epidemiological and behavioral studies done in humans (Core A), is essential to understand mechanisms underlying metabolic disease risk, and the potential effectiveness of nutritional and pharmacologic approaches to ameliorate obesity and its consequences

NORC members wishing to benefit from NORC support accessing these services please contact this Allison Xu.

Allison Xu, PhD | Core Director

 

Services Supported


Mouse body composition, food intake, and metabolism facility

Provide technical support for UCSF investigators to conduct metabolic studies using a 16-chambered Comprehensive Lab Animal Monitoring System (CLAMS), a Rodent Incubator, an EchoMRI, and Dual energy X-ray absorptiometry, which together allow measurement of food intake, water intake, motor activities, core temperature, and body composition in live mice.
 
The use of these instruments allows longitudinal measurements of key metabolic parameters in the same cohort of animals at different ages and under different feeding conditions, and will enable the accumulation of data over several months of time. We are very excited to offer access to a last generation Seahorse Bioscience XFe24 Extracellular Flux Analyzer, a platform for metabolic assays, simultaneously measuring the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a 24-well microplate, in real-time.
 

Gnotobiotic core facility 

Numerous studies have shown that the microbiome has a profound effect on metabolism. The Gnotobiotics Core provides germ-free mice, fully supported gnotobiotic experiments, and germ-free re-derivation of transgenic mouse strains.

 

Mouse genetic services

  • Genome Engineering Core: The Facility provides ES cell targeting, genomic DNA extraction from 96-well plates, expansion of targeted ES cells, mycoplasma tests, chromosome counts, preparation of ES cells for microinjection, and CRISPR/Cas9 technologies.
  • Transgenenis and Blastocyte Injection ServicesProvides services including microinjection of DNA for producing transgenic mice, microinjection of ES cells for producing gene knock-out mice, and 8 cell microinjection for producing 100% ES cell derived mouse embryos or mice. Transgenic Core work with UCSF ES Cell Targeting Core to provide a full-range of gene knock-out and knock-in services. Please contact the Genome Engineering Core for custom TALEN/CRISPR and ES cell targeting services. 
  • Cryopreservation and Rederivation Services: The cryopreservation and resuscitation services allows researchers to freeze and store embryos or sperm from research lines that they no longer wish to carry but may need to resurrect for future studies.