Michael Mcmanus, PhD

Professor
Diabetes Center
[email protected]
+1 415 476-4661

The McManus lab studies fundamental processes relating to the regulation of gene expression. We take high-throughput approaches, analyzing hundreds of thousands to millions of experiments at once, using unique and complex libraries coupled to deep sequencing. Our systems span from cell culture to in vivo models, focusing on a broad array of disease relevant tissues. From cancer to diabetes, we develop novel technologies to help us better understand how genes are regulated and how they function in cells. We aim to uncover the dark matter of the genome, to help unravel the beautiful genomic complexity of pathways and how genes interact in development and disease.

Publications: 

miR-200 deficiency promotes lung cancer metastasis by activating Notch signaling in cancer-associated fibroblasts.

Genes & development

Xue B, Chuang CH, Prosser HM, Fuziwara CS, Chan C, Sahasrabudhe N, Kühn M, Wu Y, Chen J, Biton A, Chen C, Wilkinson JE, McManus MT, Bradley A, Winslow MM, Su B, He L

Examining the evidence for extracellular RNA function in mammals.

Nature reviews. Genetics

Gruner HN, McManus MT

High-Throughput CRISPR Screening Identifies Genes Involved in Macrophage Viability and Inflammatory Pathways.

Cell reports

Covarrubias S, Vollmers AC, Capili A, Boettcher M, Shulkin A, Correa MR, Halasz H, Robinson EK, O'Briain L, Vollmers C, Blau J, Katzman S, McManus MT, Carpenter S

miR-29 Sustains B Cell Survival and Controls Terminal Differentiation via Regulation of PI3K Signaling.

Cell reports

Hines MJ, Coffre M, Mudianto T, Panduro M, Wigton EJ, Tegla C, Osorio-Vasquez V, Kageyama R, Benhamou D, Perez O, Bajwa S, McManus MT, Ansel KM, Melamed D, Koralov SB

Macrophage Exosomes Resolve Atherosclerosis by Regulating Hematopoiesis and Inflammation via MicroRNA Cargo.

Cell reports

Bouchareychas L, Duong P, Covarrubias S, Alsop E, Phu TA, Chung A, Gomes M, Wong D, Meechoovet B, Capili A, Yamamoto R, Nakauchi H, McManus MT, Carpenter S, Van Keuren-Jensen K, Raffai RL

CD81 Controls Beige Fat Progenitor Cell Growth and Energy Balance via FAK Signaling.

Cell

Oguri Y, Shinoda K, Kim H, Alba DL, Bolus WR, Wang Q, Brown Z, Pradhan RN, Tajima K, Yoneshiro T, Ikeda K, Chen Y, Cheang RT, Tsujino K, Kim CR, Greiner VJ, Datta R, Yang CD, Atabai K, McManus MT, Koliwad SK, Spiegelman BM, Kajimura S

Host-Receptor Post-Translational Modifications Refine Staphylococcal Leukocidin Cytotoxicity.

Toxins

Tromp AT, Van Gent M, Jansen JP, Scheepmaker LM, Velthuizen A, De Haas CJC, Van Kessel KPM, Bardoel BW, Boettcher M, McManus MT, Van Strijp JAG, Lebbink RJ, Haas PA, Spaan AN

BCAA catabolism in brown fat controls energy homeostasis through SLC25A44.

Nature

Yoneshiro T, Wang Q, Tajima K, Matsushita M, Maki H, Igarashi K, Dai Z, White PJ, McGarrah RW, Ilkayeva OR, Deleye Y, Oguri Y, Kuroda M, Ikeda K, Li H, Ueno A, Ohishi M, Ishikawa T, Kim K, Chen Y, Sponton CH, Pradhan RN, Majd H, Greiner VJ, Yoneshiro M, Brown Z, Chondronikola M, Takahashi H, Goto T, Kawada T, Sidossis L, Szoka FC, McManus MT, Saito M, Soga T, Kajimura S

Genomic Resolution of DLX-Orchestrated Transcriptional Circuits Driving Development of Forebrain GABAergic Neurons.

Cell reports

Lindtner S, Catta-Preta R, Tian H, Su-Feher L, Price JD, Dickel DE, Greiner V, Silberberg SN, McKinsey GL, McManus MT, Pennacchio LA, Visel A, Nord AS, Rubenstein JLR

miR-15/16 Restrain Memory T Cell Differentiation, Cell Cycle, and Survival.

Cell reports

Gagnon JD, Kageyama R, Shehata HM, Fassett MS, Mar DJ, Wigton EJ, Johansson K, Litterman AJ, Odorizzi P, Simeonov D, Laidlaw BJ, Panduro M, Patel S, Jeker LT, Feeney ME, McManus MT, Marson A, Matloubian M, Sanjabi S, Ansel KM

Thermoregulation via Temperature-Dependent PGD2 Production in Mouse Preoptic Area.

Neuron

Wang TA, Teo CF, Åkerblom M, Chen C, Tynan-La Fontaine M, Greiner VJ, Diaz A, McManus MT, Jan YN, Jan LY

Thermoregulation via Temperature-Dependent PGD2 Production in Mouse Preoptic Area.

Neuron

Wang TA, Teo CF, Åkerblom M, Chen C, Tynan-La Fontaine M, Greiner VJ, Diaz A, McManus MT, Jan YN, Jan LY

High-Complexity shRNA Libraries and PI3 Kinase Inhibition in Cancer: High-Fidelity Synthetic Lethality Predictions.

Cell reports

Mues M, Karra L, Romero-Moya D, Wandler A, Hangauer MJ, Ksionda O, Thus Y, Lindenbergh M, Shannon K, McManus MT, Roose JP

The Extracellular RNA Communication Consortium: Establishing Foundational Knowledge and Technologies for Extracellular RNA Research.

Cell

Das S, Extracellular RNA Communication Consortium, Ansel KM, Bitzer M, Breakefield XO, Charest A, Galas DJ, Gerstein MB, Gupta M, Milosavljevic A, McManus MT, Patel T, Raffai RL, Rozowsky J, Roth ME, Saugstad JA, Van Keuren-Jensen K, Weaver AM, Laurent LC

Tracing cellular heterogeneity in pooled genetic screens via multi-level barcoding.

BMC genomics

Boettcher M, Covarrubias S, Biton A, Blau J, Wang H, Zaitlen N, McManus MT

Far away from the lamppost.

PLoS biology

Oprea TI, Jan L, Johnson GL, Roth BL, Ma'ayan A, Schürer S, Shoichet BK, Sklar LA, McManus MT

Genetic Models Reveal cis and trans Immune-Regulatory Activities for lincRNA-Cox2.

Cell reports

Elling R, Robinson EK, Shapleigh B, Liapis SC, Covarrubias S, Katzman S, Groff AF, Jiang Z, Agarwal S, Motwani M, Chan J, Sharma S, Hennessy EJ, FitzGerald GA, McManus MT, Rinn JL, Fitzgerald KA, Carpenter S

miR-205 Regulates Basal Cell Identity and Stem Cell Regenerative Potential During Mammary Reconstitution.

Stem cells (Dayton, Ohio)

Lu Y, Cao J, Napoli M, Xia Z, Zhao N, Creighton CJ, Li W, Chen X, Flores ER, McManus MT, Rosen JM

Publisher Correction: Human CD45 is an F-component-specific receptor for the staphylococcal toxin Panton-Valentine leukocidin.

Nature microbiology

Tromp AT, Van Gent M, Abrial P, Martin A, Jansen JP, De Haas CJC, Van Kessel KPM, Bardoel BW, Kruse E, Bourdonnay E, Boettcher M, McManus MT, Day CJ, Jennings MP, Lina G, Vandenesch F, Van Strijp JAG, Lebbink RJ, Haas PA, Henry T, Spaan AN

A Genetic Interaction Map of Insulin Production Identifies Mfi as an Inhibitor of Mitochondrial Fission.

Endocrinology

Lee J, Pappalardo Z, Chopra DG, Hennings TG, Vaughn I, Lan C, Choe JJ, Ang K, Chen S, Arkin M, McManus MT, German MS, Ku GM

Human CD45 is an F-component-specific receptor for the staphylococcal toxin Panton-Valentine leukocidin.

Nature microbiology

Tromp AT, Van Gent M, Abrial P, Martin A, Jansen JP, De Haas CJC, Van Kessel KPM, Bardoel BW, Kruse E, Bourdonnay E, Boettcher M, McManus MT, Day CJ, Jennings MP, Lina G, Vandenesch F, Van Strijp JAG, Lebbink RJ, Haas PA, Henry T, Spaan AN

Corrigendum: A systematic comparison reveals substantial differences in chromosomal versus episomal encoding of enhancer activity.

Genome research

Inoue F, Kircher M, Martin B, Cooper GM, Witten DM, McManus MT, Ahituv N, Shendure J

Genome-wide CRISPR screen identifies FAM49B as a key regulator of actin dynamics and T cell activation.

Proceedings of the National Academy of Sciences of the United States of America

Shang W, Jiang Y, Boettcher M, Ding K, Mollenauer M, Liu Z, Wen X, Liu C, Hao P, Zhao S, McManus MT, Wei L, Weiss A, Wang H

Unexplored therapeutic opportunities in the human genome.

Nature reviews. Drug discovery

Oprea TI, Bologa CG, Brunak S, Campbell A, Gan GN, Gaulton A, Gomez SM, Guha R, Hersey A, Holmes J, Jadhav A, Jensen LJ, Johnson GL, Karlson A, Leach AR, Ma'ayan A, Malovannaya A, Mani S, Mathias SL, McManus MT, Meehan TF, von Mering C, Muthas D, Nguyen DT, Overington JP, Papadatos G, Qin J, Reich C, Roth BL, Schürer SC, Simeonov A, Sklar LA, Southall N, Tomita S, Tudose I, Ursu O, Vidovic D, Waller A, Westergaard D, Yang JJ, Zahoránszky-Köhalmi G

Unexplored therapeutic opportunities in the human genome.

Nature reviews. Drug discovery

Oprea TI, Bologa CG, Brunak S, Campbell A, Gan GN, Gaulton A, Gomez SM, Guha R, Hersey A, Holmes J, Jadhav A, Jensen LJ, Johnson GL, Karlson A, Leach AR, Ma'ayan A, Malovannaya A, Mani S, Mathias SL, McManus MT, Meehan TF, von Mering C, Muthas D, Nguyen DT, Overington JP, Papadatos G, Qin J, Reich C, Roth BL, Schürer SC, Simeonov A, Sklar LA, Southall N, Tomita S, Tudose I, Ursu O, Vidovic D, Waller A, Westergaard D, Yang JJ, Zahoránszky-Köhalmi G

The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output.

Cell stem cell

Bulut-Karslioglu A, Macrae TA, Oses-Prieto JA, Covarrubias S, Percharde M, Ku G, Diaz A, McManus MT, Burlingame AL, Ramalho-Santos M

Dual Strategies for Argonaute2-Mediated Biogenesis of Erythroid miRNAs Underlie Conserved Requirements for Slicing in Mammals.

Molecular cell

Jee D, Yang JS, Park SM, Farmer DT, Wen J, Chou T, Chow A, McManus MT, Kharas MG, Lai EC

Dual gene activation and knockout screen reveals directional dependencies in genetic networks.

Nature biotechnology

Boettcher M, Tian R, Blau JA, Markegard E, Wagner RT, Wu D, Mo X, Biton A, Zaitlen N, Fu H, McCormick F, Kampmann M, McManus MT

Drug-tolerant persister cancer cells are vulnerable to GPX4 inhibition.

Nature

Hangauer MJ, Viswanathan VS, Ryan MJ, Bole D, Eaton JK, Matov A, Galeas J, Dhruv HD, Berens ME, Schreiber SL, McCormick F, McManus MT

CRISPR/Cas-based screening of long non-coding RNAs (lncRNAs) in macrophages with an NF-κB reporter.

The Journal of biological chemistry

Covarrubias S, Robinson EK, Shapleigh B, Vollmers A, Katzman S, Hanley N, Fong N, McManus MT, Carpenter S

Defining epithelial cell dynamics and lineage relationships in the developing lacrimal gland.

Development (Cambridge, England)

Farmer DT, Nathan S, Finley JK, Shengyang Yu K, Emmerson E, Byrnes LE, Sneddon JB, McManus MT, Tward AD, Knox SM

miR-205 is a critical regulator of lacrimal gland development.

Developmental biology

Farmer DT, Finley JK, Chen FY, Tarifeño-Saldivia E, McNamara NA, Knox SM, McManus MT

A Whole-Genome RNA Interference Screen Reveals a Role for Spry2 in Insulin Transcription and the Unfolded Protein Response.

Diabetes

Pappalardo Z, Gambhir Chopra D, Hennings TG, Richards H, Choe J, Yang K, Baeyens L, Ang K, Chen S, Arkin M, German MS, McManus MT, Ku GM

MicroRNAs in ectodermal appendages.

Current opinion in genetics & development

Farmer DT, McManus MT

A systematic comparison reveals substantial differences in chromosomal versus episomal encoding of enhancer activity.

Genome research

Inoue F, Kircher M, Martin B, Cooper GM, Witten DM, McManus MT, Ahituv N, Shendure J

PIM1 kinase inhibition as a targeted therapy against triple-negative breast tumors with elevated MYC expression.

Nature medicine

Horiuchi D, Camarda R, Zhou AY, Yau C, Momcilovic O, Balakrishnan S, Corella AN, Eyob H, Kessenbrock K, Lawson DA, Marsh LA, Anderton BN, Rohrberg J, Kunder R, Bazarov AV, Yaswen P, McManus MT, Rugo HS, Werb Z, Goga A

MicroRNAs 24 and 27 Suppress Allergic Inflammation and Target a Network of Regulators of T Helper 2 Cell-Associated Cytokine Production.

Immunity

Pua HH, Steiner DF, Patel S, Gonzalez JR, Ortiz-Carpena JF, Kageyama R, Chiou NT, Gallman A, de Kouchkovsky D, Jeker LT, McManus MT, Erle DJ, Ansel KM

Biogenesis, delivery, and function of extracellular RNA.

Journal of extracellular vesicles

Patton JG, Franklin JL, Weaver AM, Vickers K, Zhang B, Coffey RJ, Ansel KM, Blelloch R, Goga A, Huang B, L'Etoille N, Raffai RL, Lai CP, Krichevsky AM, Mateescu B, Greiner VJ, Hunter C, Voinnet O, McManus MT

Identification of MiR-205 As a MicroRNA That Is Highly Expressed in Medullary Thymic Epithelial Cells.

PloS one

Khan IS, Park CY, Mavropoulos A, Shariat N, Pollack JL, Barczak AJ, Erle DJ, McManus MT, Anderson MS, Jeker LT

Choosing the Right Tool for the Job: RNAi, TALEN, or CRISPR.

Molecular cell

Boettcher M, McManus MT

Integrative analysis of 111 reference human epigenomes.

Nature

Roadmap Epigenomics Consortium, Kundaje A, Meuleman W, Ernst J, Bilenky M, Yen A, Heravi-Moussavi A, Kheradpour P, Zhang Z, Wang J, Ziller MJ, Amin V, Whitaker JW, Schultz MD, Ward LD, Sarkar A, Quon G, Sandstrom RS, Eaton ML, Wu YC, Pfenning AR, Wang X, Claussnitzer M, Liu Y, Coarfa C, Harris RA, Shoresh N, Epstein CB, Gjoneska E, Leung D, Xie W, Hawkins RD, Lister R, Hong C, Gascard P, Mungall AJ, Moore R, Chuah E, Tam A, Canfield TK, Hansen RS, Kaul R, Sabo PJ, Bansal MS, Carles A, Dixon JR, Farh KH, Feizi S, Karlic R, Kim AR, Kulkarni A, Li D, Lowdon R, Elliott G, Mercer TR, Neph SJ, Onuchic V, Polak P, Rajagopal N, Ray P, Sallari RC, Siebenthall KT, Sinnott-Armstrong NA, Stevens M, Thurman RE, Wu J, Zhang B, Zhou X, Beaudet AE, Boyer LA, De Jager PL, Farnham PJ, Fisher SJ, Haussler D, Jones SJ, Li W, Marra MA, McManus MT, Sunyaev S, Thomson JA, Tlsty TD, Tsai LH, Wang W, Waterland RA, Zhang MQ, Chadwick LH, Bernstein BE, Costello JF, Ecker JR, Hirst M, Meissner A, Milosavljevic A, Ren B, Stamatoyannopoulos JA, Wang T, Kellis M

Epigenetic and transcriptional determinants of the human breast.

Nature communications

Gascard P, Bilenky M, Sigaroudinia M, Zhao J, Li L, Carles A, Delaney A, Tam A, Kamoh B, Cho S, Griffith M, Chu A, Robertson G, Cheung D, Li I, Heravi-Moussavi A, Moksa M, Mingay M, Hussainkhel A, Davis B, Nagarajan RP, Hong C, Echipare L, O'Geen H, Hangauer MJ, Cheng JB, Neel D, Hu D, McManus MT, Moore R, Mungall A, Ma Y, Plettner P, Ziv E, Wang T, Farnham PJ, Jones SJ, Marra MA, Tlsty TD, Costello JF, Hirst M

A screen in mice uncovers repression of lipoprotein lipase by microRNA-29a as a mechanism for lipid distribution away from the liver.

Hepatology (Baltimore, Md.)

Mattis AN, Song G, Hitchner K, Kim RY, Lee AY, Sharma AD, Malato Y, McManus MT, Esau CC, Koller E, Koliwad S, Lim LP, Maher JJ, Raffai RL, Willenbring H

Systematic identification of barriers to human iPSC generation.

Cell

Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust EM, McManus MT, Song JS, Ramalho-Santos M

A high-coverage shRNA screen identifies TMEM129 as an E3 ligase involved in ER-associated protein degradation.

Nature communications

van de Weijer ML, Bassik MC, Luteijn RD, Voorburg CM, Lohuis MA, Kremmer E, Hoeben RC, LeProust EM, Chen S, Hoelen H, Ressing ME, Patena W, Weissman JS, McManus MT, Wiertz EJ, Lebbink RJ

Discovering the complexity of the metazoan transcriptome.

Genome biology

Hangauer MJ, Carpenter S, McManus MT

Massively parallel functional annotation of 3' untranslated regions.

Nature biotechnology

Zhao W, Pollack JL, Blagev DP, Zaitlen N, McManus MT, Erle DJ

Negative regulation of Hif1a expression and TH17 differentiation by the hypoxia-regulated microRNA miR-210.

Nature immunology

Wang H, Flach H, Onizawa M, Wei L, McManus MT, Weiss A

Next-generation NAMPT inhibitors identified by sequential high-throughput phenotypic chemical and functional genomic screens.

Chemistry & biology

Matheny CJ, Wei MC, Bassik MC, Donnelly AJ, Kampmann M, Iwasaki M, Piloto O, Solow-Cordero DE, Bouley DM, Rau R, Brown P, McManus MT, Weissman JS, Cleary ML

Partially penetrant postnatal lethality of an epithelial specific MicroRNA in a mouse knockout.

PloS one

Farmer DT, Shariat N, Park CY, Liu HJ, Mavropoulos A, McManus MT

The Pitx2:miR-200c/141:noggin pathway regulates Bmp signaling and ameloblast differentiation.

Development (Cambridge, England)

Cao H, Jheon A, Li X, Sun Z, Wang J, Florez S, Zhang Z, McManus MT, Klein OD, Amendt BA

Renewable RNAi.

Nature biotechnology

Blau JA, McManus MT

A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility.

Cell

Bassik MC, Kampmann M, Lebbink RJ, Wang S, Hein MY, Poser I, Weibezahn J, Horlbeck MA, Chen S, Mann M, Hyman AA, Leproust EM, McManus MT, Weissman JS

T cell activation induces proteasomal degradation of Argonaute and rapid remodeling of the microRNA repertoire.

The Journal of experimental medicine

Bronevetsky Y, Villarino AV, Eisley CJ, Barbeau R, Barczak AJ, Heinz GA, Kremmer E, Heissmeyer V, McManus MT, Erle DJ, Rao A, Ansel KM

IRE1α cleaves select microRNAs during ER stress to derepress translation of proapoptotic Caspase-2.

Science (New York, N.Y.)

Upton JP, Wang L, Han D, Wang ES, Huskey NE, Lim L, Truitt M, McManus MT, Ruggero D, Goga A, Papa FR, Oakes SA

Research resource: RNA-Seq reveals unique features of the pancreatic β-cell transcriptome.

Molecular endocrinology (Baltimore, Md.)

Ku GM, Kim H, Vaughn IW, Hangauer MJ, Myung Oh C, German MS, McManus MT

Let-7b/c enhance the stability of a tissue-specific mRNA during mammalian organogenesis as part of a feedback loop involving KSRP.

PLoS genetics

Repetto E, Briata P, Kuziner N, Harfe BD, McManus MT, Gherzi R, Rosenfeld MG, Trabucchi M

Right- and left-loop short shRNAs have distinct and unusual mechanisms of gene silencing.

Nucleic acids research

Dallas A, Ilves H, Ge Q, Kumar P, Shorenstein J, Kazakov SA, Cuellar TL, McManus MT, Behlke MA, Johnston BH

A resource for the conditional ablation of microRNAs in the mouse.

Cell reports

Park CY, Jeker LT, Carver-Moore K, Oh A, Liu HJ, Cameron R, Richards H, Li Z, Adler D, Yoshinaga Y, Martinez M, Nefadov M, Abbas AK, Weiss A, Lanier LL, de Jong PJ, Bluestone JA, Srivastava D, McManus MT

Precursor microRNA-programmed silencing complex assembly pathways in mammals.

Molecular cell

Liu X, Jin DY, McManus MT, Mourelatos Z

Widespread RNA 3'-end oligouridylation in mammals.

RNA (New York, N.Y.)

Choi YS, Patena W, Leavitt AD, McManus MT

An siRNA screen in pancreatic beta cells reveals a role for Gpr27 in insulin production.

PLoS genetics

Ku GM, Pappalardo Z, Luo CC, German MS, McManus MT

Polymerase II promoter strength determines efficacy of microRNA adapted shRNAs.

PloS one

Lebbink RJ, Lowe M, Chan T, Khine H, Wang X, McManus MT

The microRNA-processing enzyme Dicer is dispensable for somite segmentation but essential for limb bud positioning.

Developmental biology

Zhang Z, O'Rourke JR, McManus MT, Lewandoski M, Harfe BD, Sun X

Posttranscriptional silencing of effector cytokine mRNA underlies the anergic phenotype of self-reactive T cells.

Immunity

Villarino AV, Katzman SD, Gallo E, Miller O, Jiang S, McManus MT, Abbas AK

Analysis of microRNA knockouts in mice.

Human molecular genetics

Park CY, Choi YS, McManus MT

Dicer1 and miR-219 Are required for normal oligodendrocyte differentiation and myelination.

Neuron

Dugas JC, Cuellar TL, Scholze A, Ason B, Ibrahim A, Emery B, Zamanian JL, Foo LC, McManus MT, Barres BA

Dicer ablation in oligodendrocytes provokes neuronal impairment in mice.

Annals of neurology

Shin D, Shin JY, McManus MT, Ptácek LJ, Fu YH

Expanded RNA-binding activities of mammalian Argonaute 2.

Nucleic acids research

Tan GS, Garchow BG, Liu X, Yeung J, Morris JP, Cuellar TL, McManus MT, Kiriakidou M

Dicer is required for proper liver zonation.

The Journal of pathology

Sekine S, Ogawa R, Mcmanus MT, Kanai Y, Hebrok M

Lentivirus production.

Journal of visualized experiments : JoVE

Wang X, McManus M

Chd1 regulates open chromatin and pluripotency of embryonic stem cells.

Nature

Gaspar-Maia A, Alajem A, Polesso F, Sridharan R, Mason MJ, Heidersbach A, Ramalho-Santos J, McManus MT, Plath K, Meshorer E, Ramalho-Santos M

Rapid creation and quantitative monitoring of high coverage shRNA libraries.

Nature methods

Bassik MC, Lebbink RJ, Churchman LS, Ingolia NT, Patena W, LeProust EM, Schuldiner M, Weissman JS, McManus MT

LPS induces KH-type splicing regulatory protein-dependent processing of microRNA-155 precursors in macrophages.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Ruggiero T, Trabucchi M, De Santa F, Zupo S, Harfe BD, McManus MT, Rosenfeld MG, Briata P, Gherzi R

Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion.

The Journal of biological chemistry

Huang TH, Wu F, Loeb GB, Hsu R, Heidersbach A, Brincat A, Horiuchi D, Lebbink RJ, Mo YY, Goga A, McManus MT

Disruption of Dicer1 induces dysregulated fetal gene expression and promotes hepatocarcinogenesis.

Gastroenterology

Sekine S, Ogawa R, Ito R, Hiraoka N, McManus MT, Kanai Y, Hebrok M

Residual microRNA expression dictates the extent of inner ear development in conditional Dicer knockout mice.

Developmental biology

Soukup GA, Fritzsch B, Pierce ML, Weston MD, Jahan I, McManus MT, Harfe BD

Genetic analyses reveal a requirement for Dicer1 in the mouse urogenital tract.

Mammalian genome : official journal of the International Mammalian Genome Society

Pastorelli LM, Wells S, Fray M, Smith A, Hough T, Harfe BD, McManus MT, Smith L, Woolf AS, Cheeseman M, Greenfield A

Sertoli cell Dicer is essential for spermatogenesis in mice.

Developmental biology

Papaioannou MD, Pitetti JL, Ro S, Park C, Aubry F, Schaad O, Vejnar CE, Kühne F, Descombes P, Zdobnov EM, McManus MT, Guillou F, Harfe BD, Yan W, Jégou B, Nef S

Podocyte-specific deletion of dicer alters cytoskeletal dynamics and causes glomerular disease.

Journal of the American Society of Nephrology : JASN

Harvey SJ, Jarad G, Cunningham J, Goldberg S, Schermer B, Harfe BD, McManus MT, Benzing T, Miner JH

Selective miRNA disruption in T reg cells leads to uncontrolled autoimmunity.

The Journal of experimental medicine

Zhou X, Jeker LT, Fife BT, Zhu S, Anderson MS, McManus MT, Bluestone JA

Dicer1 is required for differentiation of the mouse male germline.

Biology of reproduction

Maatouk DM, Loveland KL, McManus MT, Moore K, Harfe BD

Mouse let-7 miRNA populations exhibit RNA editing that is constrained in the 5'-seed/ cleavage/anchor regions and stabilize predicted mmu-let-7a:mRNA duplexes.

Genome research

Reid JG, Nagaraja AK, Lynn FC, Drabek RB, Muzny DM, Shaw CA, Weiss MK, Naghavi AO, Khan M, Zhu H, Tennakoon J, Gunaratne GH, Corry DB, Miller J, McManus MT, German MS, Gibbs RA, Matzuk MM, Gunaratne PH

Dicer inactivation leads to progressive functional and structural degeneration of the mouse retina.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Damiani D, Alexander JJ, O'Rourke JR, McManus M, Jadhav AP, Cepko CL, Hauswirth WW, Harfe BD, Strettoi E

Conditional loss of Dicer disrupts cellular and tissue morphogenesis in the cortex and hippocampus.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Davis TH, Cuellar TL, Koch SM, Barker AJ, Harfe BD, McManus MT, Ullian EM

Dicer loss in striatal neurons produces behavioral and neuroanatomical phenotypes in the absence of neurodegeneration.

Proceedings of the National Academy of Sciences of the United States of America

Cuellar TL, Davis TH, Nelson PT, Loeb GB, Harfe BD, Ullian E, McManus MT

Michael T. McManus: Interrupting biology [interview by Hema Bashyam].

The Journal of experimental medicine

McManus MT

Members of the miRNA-200 family regulate olfactory neurogenesis.

Neuron

Choi PS, Zakhary L, Choi WY, Caron S, Alvarez-Saavedra E, Miska EA, McManus M, Harfe B, Giraldez AJ, Horvitz HR, Schier AF, Dulac C

MicroRNA expression is required for pancreatic islet cell genesis in the mouse.

Diabetes

Lynn FC, Skewes-Cox P, Kosaka Y, McManus MT, Harfe BD, German MS

Essential role for Dicer during skeletal muscle development.

Developmental biology

O'Rourke JR, Georges SA, Seay HR, Tapscott SJ, McManus MT, Goldhamer DJ, Swanson MS, Harfe BD

Lentiviral strategies for RNAi knockdown of neuronal genes.

Current protocols in neuroscience

Crittenden JR, Heidersbach A, McManus MT

Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2.

Cell

Zhao Y, Ransom JF, Li A, Vedantham V, von Drehle M, Muth AN, Tsuchihashi T, McManus MT, Schwartz RJ, Srivastava D

Duox2 exhibits potent heme peroxidase activity in human respiratory tract epithelium.

FEBS letters

Harper RW, Xu C, McManus M, Heidersbach A, Eiserich JP

RNA interference in embryonic stem cells and the prospects for future therapies.

Gene therapy

Heidersbach A, Gaspar-Maia A, McManus MT, Ramalho-Santos M

Dicer function is essential for lung epithelium morphogenesis.

Proceedings of the National Academy of Sciences of the United States of America

Harris KS, Zhang Z, McManus MT, Harfe BD, Sun X

MicroRNAs and endocrine biology.

The Journal of endocrinology

Cuellar TL, McManus MT

The microRNA miR-196 acts upstream of Hoxb8 and Shh in limb development.

Nature

Hornstein E, Mansfield JH, Yekta S, Hu JK, Harfe BD, McManus MT, Baskerville S, Bartel DP, Tabin CJ

Slowing down the Ras lane: miRNAs as tumor suppressors?

Science's STKE : signal transduction knowledge environment

Morris JP, McManus MT

TAZ, a transcriptional modulator of mesenchymal stem cell differentiation.

Science (New York, N.Y.)

Hong JH, Hwang ES, McManus MT, Amsterdam A, Tian Y, Kalmukova R, Mueller E, Benjamin T, Spiegelman BM, Sharp PA, Hopkins N, Yaffe MB

The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb.

Proceedings of the National Academy of Sciences of the United States of America

Harfe BD, McManus MT, Mansfield JH, Hornstein E, Tabin CJ

Small RNAs and immunity.

Immunity

McManus MT

MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression.

Nature genetics

Mansfield JH, Harfe BD, Nissen R, Obenauer J, Srineel J, Chaudhuri A, Farzan-Kashani R, Zuker M, Pasquinelli AE, Ruvkun G, Sharp PA, Tabin CJ, McManus MT

Cre-lox-regulated conditional RNA interference from transgenes.

Proceedings of the National Academy of Sciences of the United States of America

Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L, Jaenisch R, Jacks T

MicroRNAs and cancer.

Seminars in cancer biology

McManus MT

RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcription.

Proceedings of the National Academy of Sciences of the United States of America

Ge Q, McManus MT, Nguyen T, Shen CH, Sharp PA, Eisen HN, Chen J

A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference.

Nature genetics

Rubinson DA, Dillon CP, Kwiatkowski AV, Sievers C, Yang L, Kopinja J, Rooney DL, Zhang M, Ihrig MM, McManus MT, Gertler FB, Scott ML, Van Parijs L

Small interfering RNA-mediated gene silencing in T lymphocytes.

Journal of immunology (Baltimore, Md. : 1950)

McManus MT, Haines BB, Dillon CP, Whitehurst CE, van Parijs L, Chen J, Sharp PA

Gene silencing in mammals by small interfering RNAs.

Nature reviews. Genetics

McManus MT, Sharp PA

CP110, a cell cycle-dependent CDK substrate, regulates centrosome duplication in human cells.

Developmental cell

Chen Z, Indjeian VB, McManus M, Wang L, Dynlacht BD

Gene silencing using micro-RNA designed hairpins.

RNA (New York, N.Y.)

McManus MT, Petersen CP, Haines BB, Chen J, Sharp PA

Identification of candidate mitochondrial RNA editing ligases from Trypanosoma brucei.

RNA (New York, N.Y.)

McManus MT, Shimamura M, Grams J, Hajduk SL

Trypanosoma brucei guide RNA poly(U) tail formation is stabilized by cognate mRNA.

Molecular and cellular biology

McManus MT, Adler BK, Pollard VW, Hajduk SL

Biochemical methods for analysis of kinetoplastid RNA editing.

Methods (San Diego, Calif.)

Sabatini RS, Adler BK, Madison-Antenucci S, McManus MT, Hajduk SL

Insertional and deletional RNA editing in trypanosome mitochondria.

Nucleic acids symposium series

Hajduk SL, Adler B, Madison-Antenucci S, McManus M, Sabatini R

Research Area: 
Adipocyte and Metabolism