Human Metabolism I Mouse Metabolism and Imaging I Genetics and Genomics
Jeremy Reiter, Director
The Genetics and Genomics Core provides an array of sophisticated methods and instruments that enable human genetic and genomics research and/or the tools to generate genetic models to test hypotheses.
Services Supported by the NORC Genetics and Genomics Core:
Service Area 1: Genomics Services
Service Area 2: Viracore
Provides information, training, assistance and resources for experiments to be performed by individual investigators by providing: 1) Essential reagents, equipment, training, supervision, and monitoring of investigators wishing to ensure the proper compliance with biosafety containment required for lentiviral-based research. 2) Lentiviral preparation services for investigators. 3) Education on RNAi experimentation, through the lentiviral core website, and through protocols available at the facility.
Service Area 3: Gene Targeting
Provides high-quality ES cell services with a high probability of germline transmission. The core offers ES cell targeting, genomic DNA extraction from 96-well plates, expansion of targeted ES cells, chromosome counts, and preparation of ES cells for microinjection, to all UCSF investigators as well as external investigators.
Service Area 4: Transgenics
Provides services including microinjection of DNA for producing transgenic mice, microinjection of ES cells for producing gene knock-out mice, and 8 cell microinjection for producing 100% ES cell derived mouse embryos or mice.
Service Area 5: Cryopreservation and Resuscitation
Provide services including Sperm and Embryo Cryopreservation; Sperm and Embryo Resuscitation; and Expansion by In Vitro Fertilization. The purpose of Cryopreservation Core is to ensure: Preservation; Protection; Availability: and Sharing capacities.
Service Area 6: Rederivation
CENTER MEMBERS ARE REMINDED TO ACKNOWLEDGE THE UCSF NORC GRANT CONTRIBUTION IN THEIR PUBLICATION (P30DK098722)