Human Metabolism I Mouse Metabolism and Imaging I Genetics and Genomics
Jeremy Reiter,MD, PhD| Director
NORC members wishing to benefit from NORC support when accessing these services please contact Jeremy Reiter: [email protected]
NORC Genetics and Genomics Core provides access to, assistance with, and training in the use of an array of sophisticated methods and instruments for genetic and genomic assessment of molecular pathways underlying nutrition and obesity. As the Genetics and Genomics Core facilities are overseen by five administrative units at UCSF, this Core provides the infrastructure through which NORC users make use of those facilities. The Genetics and Genomics Core assists NORC users to identify and access those facilities. The NORC also supports the development of novel genetic and genomic technologies that are determined via a well-defined process to be of emerging need to the NORC research base.
Services Supported by the NORC Genetics and Genomics Core:
Service Area 1: Genomics Services
- Transcriptomics, single cell sequencing and high throughput sequencing: Provides key services for genetic and genomic research, including DNA extraction, SNP genotyping, gene expression, DNA and RNA sequencing, and data analysis/storage. Administrative Department: Institute of Human Genetics; Departmental Core Director: Dr. Pui-Yan Kwok
Telomere Length Measurement Service: The Telomere service provides telomere length and telomerase activity measurements. Telomere length measurement can be performed in various specimen types including leukocytes, PBMCs, isolated immune cell types, saliva, dried blood spots, buccal cells and adipose tissues. The assays use validated DNA extraction methods and high throughput quantitative PCR (qPCR).
Service Area 2: Mouse Genetics Services
Genome Engineering Core: The Facility provides ES cell targeting, genomic DNA extraction from 96-well plates, expansion of targeted ES cells, mycoplasma tests, chromosome counts, preparation of ES cells for microinjection, and CRISPR/Cas9 technologies.
Transgenenis and Blastocyte Injection Services: Provides services including microinjection of DNA for producing transgenic mice, microinjection of ES cells for producing gene knock-out mice, and 8 cell microinjection for producing 100% ES cell derived mouse embryos or mice. Transgenic Core work with UCSF ES Cell Targeting Core to provide a full-range of gene knock-out and knock-in services. Please contact http://escore.ucsf.edu/ for custom TALEN/CRISPR and ES cell targeting services.
Cryopreservation and Rederivation Services: The cryopreservation and resuscitation services allows researchers to freeze and store embryos or sperm from research lines that they no longer wish to carry but may need to resurrect for future studies.
CENTER MEMBERS ARE REMINDED TO ACKNOWLEDGE THE UCSF NORC GRANT CONTRIBUTION IN THEIR PUBLICATION (P30DK098722)