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Services


 SERVICES I TRAINING I RATE

 

Equipment Available:

Instruments within the Diabetes Center Metabolic Core include: a set of 16 metabolic chambers (Oxymax Lab Animal Monitoring System or CLAMS), two 24-chamber Food Intake Measurement Systems (FIMS), a Rodent Incubator, a couple of DEXA, an EchoMRI device and our last generation Seahorse Bioscience XFe24 Extracellular Flux Analyzer. The DEXAs, EchoMRI, Rodent Incubator, FIMS and Flux Analyzer are available for use, after training, by any UCSF and, pending availability, non-UCSF laboratories. The CLAMS is not operated by Core users.

The UCSF Diabetes Center Metabolic Core operates six kinds of equipment:

CLAMS or Metabolic chambers: http://www.colinst.com/brief.php?id=61

To measure the metabolic rates from individual animal, we use a piece of equipment made by Columbus Instruments, Inc., called CLAMS (Comprehensive Lab Animal Monitoring System). Our CLAMS unit has 16 chambers, housed in a large enclosure with precise control over the temperature and light / dark cycle. A typical CLAMS run takes 4 days and the machine can record metabolic data for up to 16 mice simultaneously. To improve the acclimation of the animals, we single-house the mice the week before the CLAMS run and we introduce them to powder food (used in a feeder in the center of the cage). They will still have access to pellets though. Make sure to transfer your animals in time if you are on a different campus.

We do not analyze data for the user, we just format them (Excel file) so you have an average per hour for each metabolic parameter (VCO2, VO2, RER, food intake,...). However, we do provide extensive information and documentation on how to interpret the CLAMS data. Right now, those values are normalized to body weight (default output from the instrument). Please note: it is very important to build a cohort of animals with similar weights (below or above 30g-35g).

 

Food Intake Measurement System: http://www.colinst.com/brief.php?id=61

Two independent systems of 24 metabolic chambers, to monitor Food Intake (cumulative amount of food eaten and bout of feeding) with food access control (fasting, paired feeding) (Columbus Instruments).

 

Rodent Incubator: http://powersscientific.com/category/insect-or-rodent-incubators/

Experiments at thermoneutrality (30 C) (Power Scientific).

 

DEXA Piximus: http://piximus.com/

Dual Energy X-ray Absorptiometry (DEXA) scanning is utilized to measure bone mineral density and tissue composition (fat mass & lean mass) in anesthetized mice. DEXA offers a relatively non-invasive method to measure body composition in small rodents, and it allows us to measure the body composition changes over time. The PIXImus DEXA scanner is ideal for longitudinal studies requiring repeated measurements on the same mouse. DEXA scans can be performed before and after compound administration to determine whether body composition has changed. Effects of genetic alterations, high fat feeding, surgical ovariectomy, etc. on body composition also can be measured. We now have two devices: one is housed within barrier (in PSB rodent Barrier facility) and the other one is house outside barrier (in the Animal Tower In-And-Out Barrier facility).

 

EchoMRI: EchoMRI-3in1™ for Live Animals (Mice), Organs, and Biopsy

http://www.echomri.com/Body_Composition_3_in_1.aspx

Echo Magnetic Resonance Imaging (EchoMRI) from Echo Medical System, Houston, TX, is used to precisely measure whole body composition parameters such as total body fat, lean mass, body fluids, and total body water in live mice without the need for anesthesia or sedation. With its high sensitivity, EchoMRI is ideal for assessing small alterations in mouse whole body composition as a result of genetic mutation or exposure to novel compounds.

 

Seahorse Bioscience XFe24 Extracellular Flux Analyzer:
http://seahorsebio.com/products/instruments/analyzers.php

The Seahorse Bioscience XFe24 Extracellular Flux Analyzer is a platform for metabolic assays, simultaneously measuring the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a 24-well microplate, in real-time.

Contact us regarding training and if you are already user, remember to sign up using the Google calendar for the Seahorse.


CENTER MEMBERS ARE REMINDED TO ACKNOWLEDGE THE UCSF NORC GRANT CONTRIBUTION IN THEIR PUBLICATION (P30DK098722)